QTL (Quantitative trait loci) that affecting main rice quality characters and Indica_Japonica differentiation were analyzed by using a recombinant inbred(RI) lines derived from a cross of Asominori×IR ₂₄ and its RFLP map with 293 molecular markers by single marker analysis and interval mapping,QTL for rice fat content and protein content was analyezed.And relation between fat content,protein content and eating taste quality traits analyzed.One QTL(fat) for fat content and three QTL(Prl,Pr2 and Pr3) for protein content were detected.It indicated that there were significant correlation between fat and cooked rice taste,cooked rice appearance.There was significant negative correlation between protein content and cooked rice eating quality.It would improve rice eating quality that increased fat content and controlled protein content.