The crossing have been done by excellent sunflower population which used for female lines and sunflower wild species with tolerance genes to Sclerotinia Sclerotiorum Lib used for male lines.The progeny of hybridization are "embryo rescue" with embryo culture technique.10 populations with tolerance to Sclerotinia Sclerotiorum Lib have been obtained.In these populations,compared with the check,the susceptible diameter decrease by 69.56%when inoculating on the disk.The susceptible rate decrease by 92.95% when inoculating in the root.In these tolerance populations,8 inbred lines with high tolerance to Sclerotinia Sclerotiorum Lib are selected by the combination method of recurrent selection and resistant identification.