适宜大豆灰斑病菌生长和产孢的培养基筛选试验
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S435.651

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Screening Test on Nutrient Mediums Suited to Cercospora sojina Hara Growing and Spore-forming
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    摘要:

    将大豆灰斑病菌(CercosporasojinaHara)在豆秸、豆荚、豆粒、豆粉、半综合、PDA、V8蔬菜汁7种培养基上培养,测定了不同培养基对菌落生长速度和产孢量的影响。结果表明,豆粒、半综合、PDA、V8蔬菜汁培养基上的菌落生长速度比其他3种快,每天平均生长4 5 6 0~5 0 96 mm2,但产孢量有很大差异。PDA和豆粒培养基产孢很少,平均每视野2 4~ 4 2个,而半综合与V8蔬菜汁培养基的产孢量较大,为2 8 4~ 30 2个。进一步对产孢量大的2种培养基从原料成本和操作程序上比较,V8蔬菜汁比半综合培养基成本低,而且操作也很简便。

    Abstract:

    The colonial growing rate and spore-forming number of Cercospora sojina Hara are different on the seven kinds of nutrient mediums such as:soybean straw,soybean pod,soybean granule,soybean powder,semi synthetic medium,PDA and V8-juice mediums.The results showed that the colonial on soybean granule,semi synthetic PDA and V8-juice medium grow more quickly than the others.The average growth is 45.60~50.96 mm2 every day,but the spore-forming number is different.The spore-forming number(28.4~30.2 spores/visual field)of the semi synthetic and V8-juice medium is greater than PDA and soybean granule medium(2.4~4.2 spores/visual field).The two kinds of mediums selected are compared in the cost and operating method.We concluded that not only the cost of V8-juice medium is cheap,but also the operating is easy too.

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宋淑云,郭文广,张伟,刘继荣.适宜大豆灰斑病菌生长和产孢的培养基筛选试验[J].东北农业科学,2002,27(S1):25-26.

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