Based on multi-variable analysis and orthogonal experimental design of regression method,mathematical model describing effects of selected parameters on experiment criterion was developed.By means of optimal calculation a set of optimum parameters for the super-fine grinding of maize pollen was achieved,with rotation speed 476r /min,grinding time 1.2h,ratio of ball and maize pollen 7,and moisture content of the maize pollen below 5%.With those parameters,100%of maize pollen was crushed and diameter of maize pollen granule was below 8μm.