花生根瘤菌高效菌株的筛选及固氮效应的研究I.花生根瘤菌高效菌株的筛选及固氮效应的盆栽试验
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S565.2

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Screening of Superior Nodule Bacteria Strains in Peanut and Their Nitrogen Fixation I.Efficiency performance in pot culture experiment
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    摘要:

    以吉林省扶余县三井子镇沙壤土为供试土壤,通过对花生根瘤菌的采集、菌株的分离、纯化和原寄主品种的回接等盆栽试验,筛选出适合吉林省不同花生品种的优良菌株(2644、2656、2697)及其组合(2644+2656、2644+2697),并对其进行生理生化特性鉴定。结果表明,2644为快生型根瘤菌,表现出相对较强的固氮酶活性,2656、2697为慢生型根瘤菌,也具有较强的固氮活性。修正了花生根瘤菌无快生型或快生型固氮活性低的传统说法。同时这3种优良菌株中两种或两种以上组合接种效果更佳,是生产上推广应用的优选菌株,这为引进优良菌种和组合接菌提供了理论依据。

    Abstract:

    Sand soil from Sanjins town of Fuyu county,Jilin province,was used as the experimental Soil.Through some pot culture experiments and field experiments such as collection of peanut nodule bacteria,strain separation and purification,and returning inoculation of original host varieties,some good strains and their combinations that suit different peanut varieties of Jlin province were selected,and the yield increasing mechanism of peanut -peanut root nodule symbiosis nitrogen fixation was clarified preliminarily.Superior nodule bacteria varieties of 2644,2656 and 2697were selected,and through pot culture experiments,their physiological and chemical characteristics were identified.The results show that2644was quick-growing type of nodule bacteria,demonstrating comparatively strong nitrogen-fixating ferment activity.2656 and 2697 were slow-growing types of nodule bacteria,with a relatively strong nitrogen fixation activity.The above results rectified the conventional opinion that peanut nodule bacteria do not have a quick-growing type or the quick-growing type has a lower nitrogen-fixating ferment activity.At the same time,a better effect could be obtained when two or more than two of the three superior strains were combined together.They were better -selected strains suitable for application in peanut production.This provides theoretical foundation for the introduction of superior nodule bacteria varieties and inoculation combination.

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吴海燕,孙淑荣,刘春光,张桂芝.花生根瘤菌高效菌株的筛选及固氮效应的研究I.花生根瘤菌高效菌株的筛选及固氮效应的盆栽试验[J].东北农业科学,2003,(6):20-25.

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  • 收稿日期:2003-08-25
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  • 在线发布日期: 2025-04-19
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