Based on multi-variable analysis and orthogonal experimental design of regression method, mathematical model describing effects of selected parameters on experiment criterion was developed. Through optimal calculation a set of optimum parameters for (dilapidating walls of maize pollen by microwave in low temperature was achieved, i.e. difference of temperature ranges 182℃, thaw time 15.7 min, repeat treatment 5 times. In this case 95 percent of maize pollen was dilapidated.