Beauverin bassiana spores with 7 diluents were separately reserved in freezer at 5℃ and indoor for 25 months. The ratio of germinating spores was 96.2%-98.2%. Spores diluted with loess, active carbon, sand, slag, turf and so on were reserved at natural temperature indoor for 19 months and the ratio of germinating spores was 90.3%-90.5%. The ratios of germinating spores were different among treatments with different diluents and reserved time. The longest valid reserve time of spores diluted with loess was 21 months, while that with corncob or legume straw power was 9 months and the control was 3 months.