The theory and characteristics of amino acid analysis techniques by integrating reverse-phase high performance liquid chromatography with pro-column derivatization were introduced in the paper. The reagents of pro-column derivatization were o-phthalaldehyde, 9 -fluorenylmethyl chloroformate, phenylisothiocyanate, 5 -N,N -dimethylaminoazobenzene -4′-sulfonyl chloride, 6-aminoquinolyl-N-hydroxy-succinimidyl carbamate and so on.