A herbicide gene（bar） was delivered into a commercial rice variety "Changbai 8" cultivated in Jilin province via biolistic method. Five fertile transgenic rice plants were obtained. Parameters of Gene Gun（PDS-1000/He） transformation of callus derived from mature embryo of Japonica rice variety were optimized by Gus gene transcient expression. When the distance between DNA-gold pellet and the target cells was 9 cm, bombard for 2 times, the transformation efficiency was the highest. It was demonstrated that bar gene had been inserted into rice genome by PCR and Southern hybridization. Three transgenic rice plants （D0-1, D0-2 and D0-3） showed herbicide resistance. Gene silence was observed in one transgenic rice plant （D0-5）.