By using Agrobacterium-mediated transformation, the callus derived from mature embryos of rice variety "Fengyou 516" had been transformed with Betaine-aladehyde dehydrogenase from Atriplex hortensis. G418 resistant plants were obtained in this study and put in rooting culture containing 5g/L NaCl respectively. By using salt tolerance selection, resistant plants were obtained. PCR molecule testing indicated that 73 transgenic plants were salt tolerance.