The growth rate of Beauveria bassiana on four medium was investigated. The results showed that the mycelium of B. bassiana grown fast in the L-broth medium, and the spores grown fast in the PDA medium. The growth of the protoplast and spores of B. bassiana were fully inhabited in the L-broth medium that containing 25 mg/L and 150 mg/L herbicide respectively, while homomycin B and other antibiotics used in this experiment had no effect on the growth of B. bassiana. The results indicated that herbicide resistant gene should be used as a selection marker gene in protoplast genetic transformation of B. bassiana.