The effect of different fertilizer treatments on nutrition was studied in a long-term fixed position fertilization experiment last for twenty years.The results showed that: the content of alkaline-hydrolysable N increased by the application of N fertilizer,the content of total P and available P increased by the application of P fertilizer,and the content of available K increased by the application of K fertilizer.After 20 years,comparing with that of pre-experiment,the alkaline-hydrolysable N increased 2.61-6.41 mg/kg in nitrogen application treatment,and it decreased 14.97-20.66 mg/kg in no nitrogen application control.Meanwhile,the total P and available P increased by 0.296-0.316 mg/kg and 48.25-53.16 mg/kg respectively by the application of P fertilizer.In the treatment of K application,the content of available K increased 129.03-154.75mg/kg,whereas in the control of no K application,it decreased 23.28-68.75 mg/kg.