Three sea buckthorn from China,Russia and Mongolia and their female and male plant were distinguished by RAPD.The results showed that female and male plant of any subspecies could be identified by one primer.For example,anyone of primer B07,A11,D03 and E03 could identify female and male plant of China sea buckthorn.Anyone of primer B06,A11 and D07 could identify female and male plant of Mongolia sea buckthorn.Anyone of primer A13,E04 and E07 could identify female and male plant of Russia sea buckthorn.Anyone of primer A13,E14,B06,B07,B10,A11,D07,E03 and E15 could identify three sea buckthorn subspecies but could not identify the sex at the same time.Using two primers,for example,A11+A13,A11+E04 or A11+E07 could identify the sex and subspecies at the same time.Genetic relationship between Russia and Mongolia sea buckthorn was rather closely because they have many same band patterns.