The salt tolerance of cotton seedling growth was studies in this experiment,using seedling of ’Handan 333’,’04264’,’Yu 15’and’409’ as materials.The height of seedling,activity of root system,leaf chlorophyll content,POD activity and the content of praline were determined and compared.The significance of difference in these indexes was analyzed to evaluate salt tolerance of seedling growth of different cotton varieties.The results showed that ’Handan 333’ was the most tolerant to salt,then is’04264’and’Yu 15’.’409’ was the least tolerant to salt.