Abstract:On the basis of a small-scale sensitivity text,the indoor virulence bioassay of Heterorhabditis indica was carried out on Dolerus tritici,and the data were processed by time-dose-mortality(TDM) model.The results showed that the LD50 of nematode strain LN2 was 1840 infective juveniles per insect larvae at 48 hours,and the LT50 of this strain were 41 hr.,44 hr.,47 hr.,51 hr.,56 hr.and 61 hr.in the dosages order of 9,000 IJ per larvae,4,500 IJ per larvae,2,250 IJ per larvae,1,130 IJ per larvae,560 IJ per larvae and 280 IJ per larvae.In conclusion,TDM model technology considers both time and dosage effects compared with Probability Analysis Technology,and adapts to the virulence evaluation of Entomopathologenic nematode.