The experiment was carried out for searching and establishing the method of culturing of myogenic satellite cells of porcine.Results demonstrated that myogenic satellite cells derived from porcine were successfully cultured with a normal tissue culture combining with enzyme digesting method and DMEM of 15% serum.The cultured cells were analyzed by morphology,chromosomes and cell growth curve.These cultured cells were typical myogenic satellite cells with normal morphology and chromosomes number.The culture and characterization of myogenic satellite cells can be provided for related experiments.