Young spike of six genotype sorghum were inoculated on YD10, L2, YLMDS and MS1 induction medium.Young spikes of six genotypes were all generated callus on MS1medium.The highest rate of 96.0% was obtained in ’05DL/5A’, whereas the lowest rate of 27.5% was obtained in ’Siza 40’.The yellow particulate callus differentiated adventitious buds.When the adventitious buds were 8cm long, they were switched to rooting media to regenerate plants.