Experiment of in vitro Gm256, a dwarf rootstock of apple, was carried out to study its tissue culture techniques which included inducing of stem tips, propagation, rooting, etc.Results showed that except proper medium dark condition was also need for the explant of GM256 to be induced to a plantlet.The proper medium for propagation was MS+3.0 mg/L BA+0.05 mg/L NAA, and the propagation rate reached 5.9%.Culture in dark condition was of no use for rooting of GM256 plantlet.The proper medium for rooting was 1/2MS+0.5 mg/LBA.