精子载体转基因技术研究进展
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S814.8

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A Review of Sperm-Mediated Gene Transfer Technology Researches
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    摘要:

    精子因在受精过程中的独特作用,而被公认为是转移外源DNA的理想载体,且操作简便,成本低廉,危险隐患相对较低,近10几年来的研究结果表明,精子载体介导法可以得到转基因阳性动物。本文对精子载体转基因方法、精子载体转基因的机理、影响精子与外源DNA结合的因素以及外源DNA在宿主动物中的存在形式等方面进行了论述,并对精子作为载体的研究方向和前景进行了预测。

    Abstract:

    Due to sperm has special function, it has been thought as ideal vectors for introducing foreign DNA.Sperm-mediated gene transformation is the most convenient way and also has little concealed damage.It is reported that several positive animals can be obtained through the sperm-mediation.Recent advance on these researches was introduced and assessed in the paper, which covered method of transgenic animal, molecular mechanism of interact sperm and foreign DNA, foreign DNA existing form of introducing animal or embryo, factors which affected integration of sperm and foreign DNA.The development tendency of sperm as vector introducing gene into animal was predicted in the paper.

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周兴,张居农.精子载体转基因技术研究进展[J].东北农业科学,2008,33(1):28-32.

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  • 收稿日期:2007-02-03
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  • 在线发布日期: 2025-01-06
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