The changes of growth and fruit yield of different cucumber varieties under four level of nitrogen, i.e., 0, 80, 240, 400kg.hm^-2 were studied.The results showed that when the nitrogen increased, the leaf area index, chlorophyll content, concentrates of petiole sap of leaves and fruits had gradually advanced.Under the 240kg.hm^-2 nitrogen level, the photosynthetic rate, stomatal conductance of cucumber reached the maximum, and the fruit yield was also the highest.’Jinqing 10’ and ’Jili Shisi’ had the consistent response under different nitrogen conditions.