Taking enzyme vitality as evaluation criterion,the culture conditions of white rot fungi were optimized and parts of function character of enzyme were studied.The results showed that the optimal conditions for White rot fungi to produce manganese peroxidase was as follows: Bran was the best source of carbon,glucose has not distinct effect to enzyme vitality;beef cream was the optimal source of nitrogen;and the best time to culture was 96 hours.The optimal conditions for white rot fungi to produce laccase were as similar as that of manganese peroxidase.The optimal pH range for manganese peroxidase was 4.4-4.8,the optimal concentrations of substrates was 1.2 mmol/L,the optimal temperature for reaction was 35℃.The optimal pH for Laccase was 4.8,the optimal concentrations of substrates was 1.2 mmol/L,the optimal temperature for reaction was 40℃.Cu²⁺ and Co²⁺ have activation to manganese peroxidase and Laccase,Fe²⁺ has some inhibition to them.However,Ag+ completely inhibit the vitality of Laccase,Mg²⁺ has little effect to the vitality of two enzymes.