In this study,a group of Charolais,Limousin,Simmental,Steppe Red Cattle,Mongolian Cattle was chosen and its population genetic polymorphism was analyzed in the level of molecule by the process of genome DNA extraction from blood,PCR amplification of 8 primers,fragment typing with polyacrylamide gel electrophoresis（PAGE）,allele and genotype analysis of every locus by UVIBAND system,calculation of allele frequencies,Polymorphism information content（PIC） and Heterozygosity by PPAP3.0 and SPSS10.0.The results showed that the number of alleles in IDVGA2,IDVGA46,TGLA44,BM1824,ETH225,BM2113,IDVGA44,IDVGA55 was 6,6,6,5,4,2,6,4,and the polymorphism information content（PIC） and H was 0.6831/0.7337,0.5963/0.6602,0.6462/0.7001,0.5581/0.6302,0.5529/0.6158,0.3711/0.4174,0.6831/0.7284,0.5431/0.6153.All of them were high polymorphic loci.So they were ideal loci in the study of looking for genetic markers related to the production performance.