Effect of using male alfalfa leaf-cutting bee on pollination of soybean CMS lines in cages was reported in the paper.The results showed that the male is an effective pollinator for soybean CMS,although the seed sets were much less than that pollinated by female.The average pod set and seed set in JLCMS8A and JLCMS17A were 35.0%,34.9% and 77.5%,70.2% of in CMS plants pollinated by female respectively.The pods and seeds per plant in JLCMS8A and JLCMS17A pollinated by male were 10.3,16.6 and 37.8,84.3 respectively.Pod set and seed set of soybean CMS plants in cages without pollinators were bellow 3%.