The results of 4 years cultural experiment showed that soil temperature was increased and soil nutrition improved by using plant vaccine technique.As a result,tomato plants were resistant to diseases and tolerant to cold stress.Input of pesticides and chemical fertilizers was cut off.Growth of tomato plants planted in greenhouse increased.Crop yield lifted by 24.75% and quality improved.Economic benefit raised by 22.9%.It was concluded that plant vaccine technique was suitable to be applied on tomato.