Transcriptional factor CBF1 was transformed into rice varieties ‘Shen6014’ and ‘Songjing No.3’ by means of agrobacterium-mediated method,and the herbicide resistance transformants were obtained after multiple selections.PCR assay revealed that the CBF1 gene was integrated in the rice genome.Compared with the control plants,seeds form T1 transgenic plants showed high germination ratio and better growth at low temperature condition.