In this study,a group of 30 Steppe Red Cattle（SRC） individuals was chosen and its population genetic polymorphism was analyzed in the level of molecule by the process of genome DNA extraction from blood,PCR amplification of 8 primers,fragment typing with polyacrylamide gel electrophoresis（PAGE）,allele and genotype analysis of every locus by UVIBAND system,calculation of allele frequencies,polymorphism information content（PIC） and Heterozygosity by PPAP3.0.The results showed that the number of alleles in IDVGA2,IDVGA46,TGLA44,BM1824,ETH225,BM2113,IDVGA44 and IDVGA55 was 5,4,3 and 4,and the polymorphism information content（PIC） was 0.5420,0.6736,0.5218 and 0.5750,respectively.These loci were high polymorphic loci.The number of alleles in BM2113,BM1824,IDVGA55 and TGLA44 was 6,6,6,4,4,2,6 and 4,and PIC was 0.7223,0.7493,0.6713,0.5849,0.6715,0.5089,0.7612 and 0.5965,respectively.They were high polymorphic loci.As genetic marker,the 8 loci can be used in the study of genetics and breeding of Grassland Red Cattle.