HAL1基因转化苜蓿再生植株及其耐盐性
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Transgenetic Alfalfa with HAL1 Gene and Its Salt Tolerance
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    摘要:

    用根癌农杆菌介导法将克隆于酵母的HAL1基因转化龙牧803苜蓿胚性愈伤组织,经2 mg/L的Basta抗性筛选及PCR检测,该基因已整合到受体基因组中,共获得了11株转基因植株。培养基耐盐性实验结果:在添加NaCl的MSO培养基上,非转基因植株在NaCl浓度高于0.6%时不能生根,逐渐死亡;转基因植株在NaCl浓度0.6%~1.0%范围内仍能生根并正常生长。由此初步证明HAL1基因已在龙牧803苜蓿中表达,并且提高了耐盐性。

    Abstract:

    The HAL1 gene cloned from yeast was introduced into alfalfa cultivar ‘Longmu 803’ em-bryo cellus.After screened by 2 mg/L of Basta and PCR,the HAL1 gene was proved inserted into the host genome and 11 transgenic plantlets were obtained.A salt-tolerance evaluation was carried out and the re-sult showed that the untransgenic plants could not root on the medium MSO with a concentration of NaCl higher than 0.6% and died gradually.Contrasted with the untransgenic plants,the transgenic plants could root in the medium with a concentration around 0.6% to 1.2% of NaCl and grew normally.This indicated that the HAL1 expressed in alfalfa and improved the salt-tolerance of alfalfa.

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刘艳芝,韦正乙,邢少辰,谭化,高明,董英山.HAL1基因转化苜蓿再生植株及其耐盐性[J].东北农业科学,2008,33(6):21-24.

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  • 收稿日期:2008-06-05
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  • 在线发布日期: 2025-01-06
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