With yeast genomic DNA as the template,HAL1 gene was amplified by PCR and ligated to the XhoI/EcoRI sites of pGEX-4T-1 vector,forming a new expression vector pGEX-HAL1.E.coli DH5α competent cells were transformed with this recombinant plasmid.The HAL1 protein was over ex-pressed in E.coli by IPTG induction.The resistance to NaCl of recombinant plasmid increased 50% com-pared with that of the control E.coli strain.About 59 kD expected protein band was separated using SDS-PAGE analysis.