Callus induction and differentiation were studied using young stem of Stachys siebordii Miq as explant and then the rooting,cutting and transplanting of tube seedling were also studied.The asexual line and rapid propagation technique of Stachys siebordii Miq were established.The results showed that the best medium for callus induction and differentiation of young stem was 1/2MS + 0.5-1.0 mg·L^-1 BA + 1.0 mg.L^-1 2,4-D and MS + 1.5 mg.L^-1 BA + 0.1 mg·L^-1 NAA,individually.The ideal medium of rooting was 1/2MS + 0.2mg·L^-1 IAA + 0.1 mg·L^-1 NAA.With cinder used as the medium,survival percent of 97% for transplantation and 92% for cutting were observed.Transplanted tube seedling was vigorous and yield of cubes increased by 18%.Root system was enlarged by 1-2 times and it wilted 7 days later in the late fall.