In this study,six media were used to evaluate seven elite maize inbred lines on their callusinduction characteristics.The results showed that ‘Huangzao 4’had the lowest callus induction rate and was unsuitable for genetic transformation.But other materials,including ‘Han 21’,‘18-599（red）’,‘8103’,‘Xianzao17’‘,501’and‘Ji853’,had high callus induction frequency and elite callus phenotypes and could be used for transgenic researches.Moreover,LM6 had been proved the best callus initiation media,because all the experimental genotypes bu‘tHuangzao 4’showed high callus induction frequency and good phenotypes.