Bio-preservative was extracted from plants,animals and micro-organisms or made by modern bio-engineering technology,which were more and more concerned by researchers due to their safety,healthy and efficiency.The definition,principles and characteristics of bio-preservative and multiplex bio-preservative were expounded in the paper.The current situation of application of bio-preservative was introduced in detail from plant-derived,animal-derived,microbial-derived and enzyme.Multiplex bio-preservative on preservation for aquatic products was proposed basing on the characteristics of bio-preservative and hurdle technology,the feasibility of preservation combined with low temperature preservation and modified atmosphere packaging was analyzed.It was clear that the combination of bio-preservative and other technology effectively could greatly prolong shelf life of aquatic products.