Extraction of intact RNA is essential for gene expression analysis.The isolation of high-quality RNA from adipose tissue is difficult due to numerous lipids.In this paper,we reported a protocol which we have optimized based on the Invitrogen TRIzol procedures to produce high yields of purified fully intact RNA from broilers abdominal adipose.The total RNA isolated using this procedure demonstrated sharp,undegraded 28S,18S,and 5S ribosomal RNA bands.The average ratio OD260/OD280 and concentration of RNA were 1.893 and 263 μg/mL,respectively.Successful RNA extraction was also observed by heat preservation experiment.RT-PCR analysis of chicken LPL and GAPDH genes was used to verify the quality of the RNA.