28 SSR markers were used to evaluate the genetic diversity of 32 wild soybean accessions collected from Longjing Conserved Region in Jilin Province.A total of 120 polymorphic bands were amplified with these SSR markers and the average alleles were 4.29 for each marker.The coefficient of genetic similarity among 32 wild soybean accessions ranged from 0.58 to 1.0,the average genetic similarities coefficient was 0.63.Clustering analysis showed that there was a relationship between growth tendency and geographic proximity,and the phenomenon of genetic plaque could be seen clearly.The distribution of Simpson index for 28 SSR loci was 0.1191 ～0.6738,the average Simpson index were 0.4544;Shannon-weaver index for 28 SSR loci was 0.2771～1.4783,the average Shannon-weaver index were 0.8865.These results showed that there existed a highly genetic diversity for wild soybean accessions from Longjing area in Jilin Province.