Using four-factor and five-level quadratic rotational regression combination design and taking soybean fat as goal,taking density and application rate of nitrogen,phosphorus and potassium as research test factors,the influences of different fertilizer application and density of crop to the yield of the middle-late-maturing soybean cultivar ‘Changnong 18’ has been studied in the experiment.The mathematical models of the fat and fertilizer application and density of crop have been established and analyzed.The ideal match pattern of various factors and their suitable value scope has been pointed out.The comprehensive cultivation measures for cultivation of high fat of soybean cultivar ‘Changnong 18’was obtained.This provided reference for high-fat soybean cultivation in the central region of Jilin Province.