Swine influenza,caused by Orthomyxoviridae Division swine influenza viruses,is an acute,fever and a high degree of contact,and repeated respiratory disease.The clinical exhibition characterized the rapid onset,high fever cough,nasal discharge from the breathing difficulties,failure,and pregnant swine reproductive failure,abortion,death and so on.At present,the swine influenza widespread occurred in China and around and brought huge economic losses and serious harm to human health.It is brief overview on the swine influenza diagnostic techniques of molecular biology in the paper,to provide the reference of timely diagnosis of swine influenza.