Different extraction methods of auricularia polysaccharide were studied in this paper.The results showed that the ultrasonic synergistic combination of enzyme and high-pressure hot water extraction could significantly increase the extraction rate of polysaccharides.The optimal extraction conditions were as follows: the extraction solvent ratio was 50 and the ultrasonic power was 120W;the amount of complex enzyme consisting of pectinase and cellulase used was 1.5% with a reaction time of 80min at 50℃ and pH5.The amount of protease used was 2.0% with a reaction time of 60min at 50℃and pH6,and the extraction time of high-pressure hot water extraction was 80min at 115℃.Auricularia polysaccharide extraction rate was 16.82%.