In this study,comparative investigation on the competitive ability,reproduction ability of a high oleic acid GM soybean line ‘HOA80’, recipient variety ‘SW80 ’and a non-GM cultivated variety ’Jiyu 71 ’was conducted in the natural agro-ecological environment.The results showed that ‘ HOA80 ’exhibited similar competitiveness and reproduction as that of ‘SW80 ’and ‘Jiyu 71 ’in a favorable situation.All three varieties tested exhibited slower growth rate,less soil coverage,unable to mature and set seed when planted under the uncultivated soil.While been sowed at the surface of the same type of soil,all seeds could not even germinate.It is concluded that the high oleic soybean line ‘HOA80 ’is likely to present the lowest weediness potential in Jilin agro-ecological environment when imported as raw materials for industrial processing.