高效溶磷微生物菌株的筛选、鉴定及其对磷素效率的影响
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S154

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吉林省科技厅重点项目(20080254)


Isolation and Identification of Phosphate Solubilizing Microorganisms and Their Effect on Utilization of Phosphorus
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    摘要:

    从我省中部地区种植玉米的贫磷土壤样品进行分离筛选获得了综合能力较强的细菌24株;通过DNA测序和16S rRNA系统发育分析,分别鉴定为枯草芽孢杆菌(Bacillus subtilis strain Q1)、苏云金芽胞杆菌(Bacillus thuringiensis serovar kurstaki strain AR-10)、荧光假单胞菌(Pseudomonas fluorescens Pf0-1)、蜡状芽孢杆菌(Bacillus cereus)、伯克金氏菌(Burkholderia sp.CEB01056)、Oxalobacteraceae bacterium NR186和肠杆菌(Enterobacter ludwigii strain K9);田间试验结果表明:溶磷发酵液与化肥配施的情况下,磷肥用量减少1/3或2/3的处理与全量磷肥产量差异不显著,各个处理与对照相比均表现明显的增产效应。

    Abstract:

    Twenty-four strains of lytic-enzymes producing bacteria were isolated from the phosphate -lacked soil in the middle of Jilin province.Among them,seven strains which have strongest phosphate -solubilizing activities were identified as Bacillus subtilis strain Q1,Bacillus thuringiensis serovar kurstaki strain AR-10,Pseudomonas fluorescens Pf0-1,Bacillus cereus,Burkholderia sp.CEB01056, Oxalobacteraceae bacterium NR186 and Enterobacter ludwigii strain K9 according to 16s rRNA gene sequence. No significant yield reduction was found in the treatment which was amended with 33%and 66% phosphate fertilizer compared to full-amended treatment and all the three amendment showed significant increment in the yield compared to control.

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金荣德,范作伟,高星爱,张爱平,吴海燕.高效溶磷微生物菌株的筛选、鉴定及其对磷素效率的影响[J].东北农业科学,2011,36(1):13-16.

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  • 收稿日期:2010-12-24
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  • 在线发布日期: 2024-12-07
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