White worms of Ostrinia furnacalis were collected from fields in Jilin Province the isolation and purification of new Beauveria bassiana strains were undertook.7 strains（D 1worm-5,D 1 worm-9,L 2 worm-8,L1-1-1,D6 worm-2,D10 worm-2,S9 worm-X-1） were obtained,which showed high sporulation,germination rate and virulence against larva of Ostrinia furnacalis.The results illustrated that the sporulation of all 7 strains were between 16-33.5 billions spores /g by solid fermentation,the germination rate of spores were 48%-93% at 16 hours and 100% at 22 hours post cultivation,respectively.The muscardine lethal rate was 93%-100% at a 10.6 billions /ml spores density.The virulence was not correlated to sporulation and germination rate,but positively correlated to spores density and acting time.