Tissue culture and regeneration of the main cultivars of flax in Jilin province was investigated in this study and regenerated plants were obtained.Research results were for cultivar’Jiya No.4’,adventitious buds regenerated from upper and lower part of hypocotyls derived from sterile seedlings with a ratio of 47% and 90%,respectively.The bud status was affected by IAA.The optimal concentration of IAA was 1.0mg/L.100% of adventitious bud induction rate and less vitrification rate of explants was gotten on medium with 1.0mg/l IAA and 0.5mg/l 6-BA.Shoots regenerated was moved to rooting medium when they grow to approximate 2cm,and 80% of the regenerated plants survived when they were transplanted to soil.There was no significant difference among different cultivars and the adventitious bud rate could up to 90%.However,vitrification rate were rather different among varieties and the shoots from vitrified explants were vitrified and could not grow normally.