In this study,conditions for the conjugal transfer of pSET152 from E.coli ET12567（pUZ8002） to Streptomyces ahygroscopicus gongzhulingensis were established.The highest frequency was obtained on MS medium.The frequency of conjugant formation was 1.03×10^-6～1.23×10^-5 depending on heat treatment of the spores at 50 ℃ for 10 min,donor and recipient cells mixed in a 1:10 ratio,incubated 16 to 18 hours and then covered with antibiotics.After five generations selection under stress conditions,it was demonstrated by PCR experiments that plasmid pSET152 has integrated into chromosomes of the recipient strains.And this is the first report on the transfer system of S.gongzhulingensis.