Two methods were applied in the study that used different chemical reagents（Ethyl acetate,Water-saturated N-butanol） to extract aquatic extracts from cultivated ginseng soil and directly used different chemical reagents（Ethyl acetate,N-butanol,Methanol,Distilled water） to extract cultivated ginseng soil.Activity of each constituent was determined with ginseng seeds.Root vigor and MDA content of ginseng seedling was determined after treated with higher activity constituents.The results showed that each constituent of extracts from cultivated ginseng soil had effects on radicles growth of ginseng seeds in various degree,and inhibited effects were strengthened with concentration increasing.Both ethyl acetate phase of aquatic extracts and ethyl acetate extracts from cultivated ginseng soil manifested stronger inhibited effects,and both constituents had significant effects on root vigor and MDA content of ginseng seedling.The content of MDA increased 23.8% and 37.5%,respectively,which was dramatically different from that of control（P＜0.01）.The root vigor decreased 17.2% and 27.1%,respectively,which was significantly lower than that of control（P＜0.01）.Thus,it is an effective approach that we extract cultivated ginseng soil with ethyl acetate to gain a part of ginseng allelochemicals,which could promote root cell membrane lipid peroxidation and increase membrane permeability,and reduce root activity of ginseng seedling and influence its growth and development.