Growth characteristics of the Klebsiellasp.2N3 strain in chlorimuron-ethyl were examined in the paper.The results showed that when glucose was carbon source,5×10^-3 g/L chlorimuron-ethyl was degraded completely after 96 hours,while only 5% of chlorimuron-ethyl was degraded when peptone was the nitrogen source.So glucose was the best carbon source.Optimal environment for the growth orthogonal test of 2N3 showed that concentration of glucose was 5%,the inoculate of 2N3 1%,sodium chloride 0.2%,optimal ventilation 100 mL,after inoculating 18 h,OD₆₀₀ was up to 0.8 and growth of 2N3 was the most.