Eight soybean varieties of high protein content or high yield were selected and six crosses were made and protein content of F₅ generation was analyzed.The results showed that the protein content of F₅ generation was positively correlated with that of parent especially female parent.CV% of high protein content combination was lower than that of combination between high protein content variety and high yield ones.The protein content of F₅ generation was negatively correlated with yield per plant,but it was positively correlated with the 100-grain weight.