The citrate synthase gene was transformed into main cultivar（super rice’Jijing 88’） of Jilin Province,mediated with Agrobacterium tumefaciens.Total 162 obtained transgenic plants（T0） were validated by PCR and southern blot.Five transgenic plants（T₃） with tolerance to low phosphorus in soil and good agronomic characteristics were obtained by PPT selection,PCR and Southern blot confirmation and tolerance test to low phosphorus.The citrate synthase activity and citrate acid contents in root tips and leaves of the 5 transgenic plants were higher than the control.The growth and yield of the transgenic plants were superior to the control.