Two strains of bacteria（CJ1,CJ2） and fungi（ZJ1,ZJ2） were isolated from the lead-contaminated soil to investigate their efficacy in decrease lead-contamination.After liquid cultivation in the medium containing high levels of lead,the lead removal rate of each strain were reached at 60.6%,51.3%,78.7% and 47.2%,respectively.Then,two strains（CJ1 and fungi ZJ1） which showed the highest removal rate were selected for further study.Two strains,ryegrass,EDDS（ethylenediamine succinic acid） and chelating agent was combined at different composition to study their efficacy in lead uptake.It was found that combining EDDS with ZJ1 showed the best efficacy and the lead content in the roots and leaves of ryegrass were increased by 7.7 times and 10.68 times compared to control,respectively.In a conclusion,the application of ryegrass with combined treatment would stimulate lead uptake by absorption and transmission.