The field experiment was carried out for studying effect of raising rice seedlings with non-soil substrate.The results indicated that the seedling quality was notable better in raising seedlings with non-soil substrates than that of the control;MDA of rice seedlings in vivo was decreased,SOD,CAT and AsA-POD activities of protective enzymes were significantly increased.These made the seedlings growing better.The reproductive process of the rice was significantly promoted and the rice yield increased by 5.91% compared with the control.Therefore,raising seedlings with non-soil substrates could replace soil substrates.It supplied a new seedling raising pattern for rice cultivation.