240 1-day-old Saibei Silkies were divided into 4 groups randomly,60 for every group in average.They were intranasal inoculated with 2NDV（Newcastle Disease Vaccine）,3NDV,4NDV,5NDV on 35 days in the same breeding conditions and basic immunization.The antibody titer and immune organ index was determined.The results showed that the antibody titer and the immune organs index of 3NDV inoculation were significantly higher than the other groups.It was appropriate for Saibei slikies.