Taking Vitis amurensis Rupr.Var.’Zuohongyi’ as test materials and applying HPLC-MS/MS technology,varieties and changes of anthocyanins content in fruit development were studied.The results showed that there was no anthocyanins accumulation in peel before turning color phase.As the fruit matured,anthocyanin content increased gradually and reached the most when fruit picked.13 kinds of anthocyanins had been detected in fruit development of Vitis amurensis.,in which 6 anthocyanins were diglucosides and 7 anthocyanins were monoglucosides.