The stems of Actinidia Qinmei were taken as explants and induced callus formation,and then the calli were placed on different combinations regeneration medium.The different concentrations of Cu²⁺ were added into the media to screen optimal concentrations of Cu²⁺ on regeneration and rooting of Kiwifruit callus.The results showed that the regeneration and rooting rate of Kiwifruit callus can be increased distinctly on the medium with Cu²⁺ content of 1.0 mg·L-1.But the effects were decreased significantly on the medium with more than Cu²⁺ content of 2.0 mg·L-1.